Abstract: Assisted hatching is a laboratory technique makes an artificial opening in thick zona to make the embryonic cells can hatch out and attach to the uterine wall and form pregnancy. Laser assisted hatching used in conjugation with intra-cytoplasmic insemination fertilization. Material and methods: The study was conducted at Kamal al-Samarrai Specialist Hospital of fertility and IVF-Baghdad during the period from 3rd July 2016 to 10th November 2017. Fifty nine infertile couples have been enrolled in this study and were included in ICSI cycle. The patients subdivided into 3 groups according to the duration of laser exposure (2, 4.5 and 8 μs). Results: younger women had higher successful clinical pregnancy compared to older women (40% vs. 27.6%), 4.5 μs had the highest successful clinical pregnancy 57.9% compared to 2 μs and 8 μs, with an PR (95%CI) 8.25 (1.797 – 37.881). Conclusion: Age has modest effect to predict successful clinical pregnancy outcome, in which the older the age of women the lower probability of clinical pregnancy, in multivariate analysis 4.5 μs (and consequently 6.1 μm) was 7 folds associated with better clinical pregnancy compared to 8 μs.
Keywords: Assisted Hatching Technique, Clinical Pregnancy, Diode Laser