{"id":18302,"date":"2021-12-18T13:13:59","date_gmt":"2021-12-18T10:13:59","guid":{"rendered":"https:\/\/bccru.uobaghdad.edu.iq\/?p=18302"},"modified":"2021-12-20T09:22:07","modified_gmt":"2021-12-20T06:22:07","slug":"effect-of-leishmania-major-infection-on-the-expression-of-tgf-beta-in-murine","status":"publish","type":"post","link":"https:\/\/bccru.uobaghdad.edu.iq\/?p=18302","title":{"rendered":"Effect of Leishmania major Infection on the Expression of TGF Beta in Murine"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_wp_text]<\/p>\n<h4><\/h4>\n<h3 style=\"text-align: center;\"><a href=\"https:\/\/archrazi.areeo.ac.ir\/article_124710.html\">Effect of Leishmania major Infection on the Expression of TGF Beta in Murine<\/a><\/h3>\n<h4 style=\"text-align: center;\">Al-Muhsin Al-Khayat, Ahmed Kalef, Mohammed Khashman,<br \/>\n1. Department of Basic Sciences, College of Dentistry, University of Baghdad, Baghdad, Iraq<br \/>\n2. Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Iraq<br \/>\n3. National Cancer Research Centre, University of Baghdad, Iraq<\/h4>\n<h4><\/h4>\n<h4><\/h4>\n<h4 style=\"text-align: left;\">Abstract<\/h4>\n<div class=\"padding_abstract justify\" style=\"text-align: left;\"><em>Leishmania major<\/em>\u00a0is a protozoan parasite that causes cutaneous Leishmaniasis disease in human beings and animals. The disease is prevalent in tropical and semitropical countries and has great health importance. The present study aimed to identify the histological changes in the organs infected with\u00a0<em>L. major\u00a0<\/em>and to provide a sophisticated diagnostic method for infection through detecting TGF-\u03b2 cytokine by immunohistochemistry technique(IHC) from October 2020 to January 2021. A total of 40 samples of paraffin blocks were used for different organs including skin, spleen, liver, kidney, and heart of male and female BALB\/c mice, aged 6-8 weeks, which were previously infected subcutaneously with\u00a0<em>L. major\u00a0<\/em>promastigotes at a dose of 1\u00d710<sup>7\u00a0<\/sup>promastigotes\/moues. The result indicated epidermal hyperplasia with diffuse severe lymphohistiocytic inflammatory cells infiltration in the dermis. Hyperplasia of the lymphoid follicles was observed in infected spleen and scattered polymorphonuclear cells mainly neutrophil masses with a random distribution of microgranulomas foci composed of lymphocytes and macrophages within the liver parenchyma around central veins and portal areas. The infected kidney showed aggregation of perivascular mononuclear cells (lymphocytes and macrophages) in the renal cortex. Mononuclear lymphocytes and macrophages were observed within the heart parenchyma especially around blood vessels. Additionally, evaluation of TGF-\u03b21 expression was highly strong for skin, spleen, relatively strong for liver, heart, and weak for the kidney. In conclusion, infection was accompanied by clinical and histological changes as well as inflammatory diseases. Furthermore, the determination of TGF-\u03b2 expression level depends on the diagnosis of infection. A clear understanding of immune mechanisms is essential for preventing, treating, and controlling strategies of this infection.<\/div>\n<div style=\"text-align: left;\"><\/div>\n<div>\n<p style=\"text-align: left;\">\n<\/div>\n<p>[\/vc_wp_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_wp_text] Effect of Leishmania major Infection on the Expression of TGF Beta in Murine Al-Muhsin Al-Khayat, Ahmed Kalef, Mohammed Khashman, &#8230; <a class=\"cz_readmore\" href=\"https:\/\/bccru.uobaghdad.edu.iq\/?p=18302\"><i class=\"fa fa-angle-left\" aria-hidden=\"true\"><\/i><span>\u0627\u0644\u0645\u0632\u064a\u062f<\/span><\/a><\/p>\n","protected":false},"author":43,"featured_media":18311,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"no","_lmt_disable":"","footnotes":""},"categories":[10],"tags":[],"class_list":["post-18302","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-10"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Effect of Leishmania major Infection on the Expression of TGF Beta in Murine | \u0627\u0644\u0645\u0631\u0643\u0632 \u0627\u0644\u0648\u0637\u0646\u064a \u0627\u0644\u0631\u064a\u0627\u062f\u064a \u0644\u0628\u062d\u0648\u062b \u0627\u0644\u0633\u0631\u0637\u0627\u0646<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link 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