NUCLEAR TARGETING OF LATENT MEMBRANE PROTEIN 1 OF EPSTEIN BARR VIRUS IN TISSUES FROM PATIENTS WITH PANCREATIC CARCINOMA

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م. باسم محمد، ا.م.د. كفاح عبد الغفور، أ.د. سعد محمد و خليل اسماعيل

Biochemical and Cellular Archives, Vol.18, Supplement 1, pp. 1293-1297, 2018

ABSTRACT: Many studies have implicated EBV in the pathogenesis as well as carcinogenesis of a relevant range of malignant squamous cell carcinomas. The present retrospective study was designed to: (1) investigate the frequency of EBV infections associated with the pancreatic carcinoma, (2) explore the impact this virus on the expressed histopathological grades and the stages of those cancers; and (3) screen the expressedimmunohistochemical– EBV Latent Membrane Protein-1 reactions, as referred to signal scores and intensities of these reactions in the tested specimens. The current retrospective study enrolled a number of 29 paraffinized pancreatic tissues from the archives of the period 2011- 2017 of the major hospitals and many private histopathological laboratories in Baghdad. The research included 19 pancreatic carcinomatous tissues as well as 10 biopsies from an apparently normal pancreatic tissues. Each 4 mm – thick sections of the requested tissue block was stuck on a positive charged slide to be used for an immunohistochemical (IHC) technique for detecting EBV Latent Membrane Protein 1 using monoclonal primary antibodies against them. Histopathological examination revealed that well, moderate and poor grades of pancreatic carcinomas constituted 21%, 63% and 16%, respectively. The EBV- LMP1 was detected in 42% (8 out of 19) of pancreatic cancerous tissues. Seven out of eight tissues with positive EBV-LMP1 reactions have moderate differentiated grade, while only one tissue (1 out of 8) have well-differentiated grade and no expression of poor differentiation. It could possibly point that EBV infections in pancreatic carcinomas could have an initiating and/or cofactor roles, in collaboration with other important oncogenic factors or agents in the pancreatic carcinogenesis which could happened as an early- event along other molecular attacks in this process.
Key words : Epstein Bar Virus, Latent Membrane Protein 1, immunohistochemistry, pancreatic carcinoma.